Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa

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dc.contributor.authorBernardo, Pauline
dc.contributor.authorMuhire, Brejnev
dc.contributor.authorFrançois, Sarah
dc.contributor.authorDeshoux, Maëlle
dc.contributor.authorHartnady, Penelope
dc.contributor.authorFarkas, Kata
dc.contributor.authorKraberger, Simona
dc.contributor.authorFilloux, Denis
dc.contributor.authorFernandez, Emmanuel
dc.contributor.authorGalzi, Serge
dc.contributor.authorFerdinand, Romain
dc.contributor.authorGranier, Martine
dc.contributor.authorMarais, Armelle
dc.contributor.authorBlasco, Pablo Monge
dc.contributor.authorCandresse, Thierry
dc.contributor.authorEscriu, Fernando
dc.contributor.authorVarsani, Arvind
dc.contributor.authorHarkins, Gordon W
dc.contributor.authorMartin, Darren P
dc.contributor.authorRoumagnac, Philippe
dc.date.accessioned2016-08-15T13:21:55Z
dc.date.available2016-08-15T13:21:55Z
dc.date.issued2016
dc.date.updated2016-08-12T14:11:56Z
dc.description.abstractLittle is known about the prevalence, diversity, evolutionary processes, genomic structures and population dynamics of viruses in the divergent geminivirus lineage known as the capulaviruses. We determined and analyzed full genome sequences of 13 Euphorbia caput-medusae latent virus (EcmLV) and 26 Alfalfa leaf curl virus (ALCV) isolates, and partial genome sequences of 23 EcmLV and 37 ALCV isolates. While EcmLV was asymptomatic in uncultivated southern African Euphorbia caput-medusae, severe alfalfa disease symptoms were associated with ALCV in southern France. The prevalence of both viruses exceeded 10% in their respective hosts. Besides using patterns of detectable negative selection to identify ORFs that are probably functionally expressed, we show that ALCV and EcmLV both display evidence of inter-species recombination and biologically functional genomic secondary structures. Finally, we show that whereas the EcmLV populations likely experience restricted geographical dispersion, ALCV is probably freely moving across the French Mediterranean region.en_ZA
dc.identifierhttp://dx.doi.org/ 10.1016/j.virol.2016.03.016
dc.identifier.apacitationBernardo, P., Muhire, B., François, S., Deshoux, M., Hartnady, P., Farkas, K., ... Roumagnac, P. (2016). Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa. <i>Virology</i>, http://hdl.handle.net/11427/21250en_ZA
dc.identifier.chicagocitationBernardo, Pauline, Brejnev Muhire, Sarah François, Maëlle Deshoux, Penelope Hartnady, Kata Farkas, Simona Kraberger, et al "Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa." <i>Virology</i> (2016) http://hdl.handle.net/11427/21250en_ZA
dc.identifier.citationBernardo, P., Muhire, B., François, S., Deshoux, M., Hartnady, P., Farkas, K., ... & Ferdinand, R. (2016). Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa. Virology, 493, 142-153.en_ZA
dc.identifier.issn0042-6822en_ZA
dc.identifier.ris TY - Journal Article AU - Bernardo, Pauline AU - Muhire, Brejnev AU - François, Sarah AU - Deshoux, Maëlle AU - Hartnady, Penelope AU - Farkas, Kata AU - Kraberger, Simona AU - Filloux, Denis AU - Fernandez, Emmanuel AU - Galzi, Serge AU - Ferdinand, Romain AU - Granier, Martine AU - Marais, Armelle AU - Blasco, Pablo Monge AU - Candresse, Thierry AU - Escriu, Fernando AU - Varsani, Arvind AU - Harkins, Gordon W AU - Martin, Darren P AU - Roumagnac, Philippe AB - Little is known about the prevalence, diversity, evolutionary processes, genomic structures and population dynamics of viruses in the divergent geminivirus lineage known as the capulaviruses. We determined and analyzed full genome sequences of 13 Euphorbia caput-medusae latent virus (EcmLV) and 26 Alfalfa leaf curl virus (ALCV) isolates, and partial genome sequences of 23 EcmLV and 37 ALCV isolates. While EcmLV was asymptomatic in uncultivated southern African Euphorbia caput-medusae, severe alfalfa disease symptoms were associated with ALCV in southern France. The prevalence of both viruses exceeded 10% in their respective hosts. Besides using patterns of detectable negative selection to identify ORFs that are probably functionally expressed, we show that ALCV and EcmLV both display evidence of inter-species recombination and biologically functional genomic secondary structures. Finally, we show that whereas the EcmLV populations likely experience restricted geographical dispersion, ALCV is probably freely moving across the French Mediterranean region. DA - 2016 DB - OpenUCT DP - University of Cape Town J1 - Virology LK - https://open.uct.ac.za PB - University of Cape Town PY - 2016 SM - 0042-6822 T1 - Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa TI - Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa UR - http://hdl.handle.net/11427/21250 ER - en_ZA
dc.identifier.urihttp://hdl.handle.net/11427/21250
dc.identifier.vancouvercitationBernardo P, Muhire B, François S, Deshoux M, Hartnady P, Farkas K, et al. Molecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africa. Virology. 2016; http://hdl.handle.net/11427/21250.en_ZA
dc.languageengen_ZA
dc.publisherElsevieren_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_ZA
dc.sourceVirologyen_ZA
dc.source.urihttp://www.sciencedirect.com/science/journal/00426822
dc.subject.otherGeminiviridae
dc.subject.otherPrevalence
dc.subject.otherRecombination
dc.subject.otherAlfalfa leaf curl virus
dc.subject.otherGenome organization
dc.subject.otherFrance
dc.titleMolecular characterization and prevalence of two capulaviruses: Alfalfa leaf curl virus from France and Euphorbia caput-medusae latent virus from South Africaen_ZA
dc.typeJournal Articleen_ZA
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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