Microbial solvent formation revisited by comparative genome analysis

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dc.contributor.authorPoehlein, Anja
dc.contributor.authorSolano, José D M
dc.contributor.authorFlitsch, Stefanie K
dc.contributor.authorKrabben, Preben
dc.contributor.authorWinzer, Klaus
dc.contributor.authorReid, Sharon J
dc.contributor.authorJones, David T
dc.contributor.authorGreen, Edward
dc.contributor.authorMinton, Nigel P
dc.contributor.authorDaniel, Rolf
dc.contributor.authorDürre, Peter
dc.date.accessioned2017-04-04T06:25:52Z
dc.date.available2017-04-04T06:25:52Z
dc.date.issued2017-03-09
dc.date.updated2017-03-09T19:03:16Z
dc.description.abstractBackground: Microbial formation of acetone, isopropanol, and butanol is largely restricted to bacteria belonging to the genus Clostridium. This ability has been industrially exploited over the last 100 years. The solvents are important feedstocks for the chemical and biofuel industry. However, biological synthesis suffers from high substrate costs and competition from chemical synthesis supported by the low price of crude oil. To render the biotechnological production economically viable again, improvements in microbial and fermentation performance are necessary. However, no comprehensive comparisons of respective species and strains used and their specific abilities exist today. Results: The genomes of a total 30 saccharolytic Clostridium strains, representative of the species Clostridium acetobutylicum, C. aurantibutyricum, C. beijerinckii, C. diolis, C. felsineum, C. pasteurianum, C. puniceum, C. roseum, C. saccharobutylicum, and C. saccharoperbutylacetonicum, have been determined; 10 of them completely, and compared to 14 published genomes of other solvent-forming clostridia. Two major groups could be differentiated and several misclassified species were detected. Conclusions: Our findings represent a comprehensive study of phylogeny and taxonomy of clostridial solvent producers that highlights differences in energy conservation mechanisms and substrate utilization between strains, and allow for the first time a direct comparison of sequentially selected industrial strains at the genetic level. Detailed data mining is now possible, supporting the identification of new engineering targets for improved solvent production.
dc.identifier.apacitationPoehlein, A., Solano, J. D. M., Flitsch, S. K., Krabben, P., Winzer, K., Reid, S. J., ... Dürre, P. (2017). Microbial solvent formation revisited by comparative genome analysis. <i>Biotechnol Biofuels</i>, http://hdl.handle.net/11427/24143en_ZA
dc.identifier.chicagocitationPoehlein, Anja, José D M Solano, Stefanie K Flitsch, Preben Krabben, Klaus Winzer, Sharon J Reid, David T Jones, et al "Microbial solvent formation revisited by comparative genome analysis." <i>Biotechnol Biofuels</i> (2017) http://hdl.handle.net/11427/24143en_ZA
dc.identifier.citationPoehlein, A., Solano, J. D. M., Flitsch, S. K., Krabben, P., Winzer, K., Reid, S. J., ... & Dürre, P. (2017). Microbial solvent formation revisited by comparative genome analysis. Biotechnology for Biofuels, 10(1), 58.
dc.identifier.ris TY - Journal Article AU - Poehlein, Anja AU - Solano, José D M AU - Flitsch, Stefanie K AU - Krabben, Preben AU - Winzer, Klaus AU - Reid, Sharon J AU - Jones, David T AU - Green, Edward AU - Minton, Nigel P AU - Daniel, Rolf AU - Dürre, Peter AB - Background: Microbial formation of acetone, isopropanol, and butanol is largely restricted to bacteria belonging to the genus Clostridium. This ability has been industrially exploited over the last 100 years. The solvents are important feedstocks for the chemical and biofuel industry. However, biological synthesis suffers from high substrate costs and competition from chemical synthesis supported by the low price of crude oil. To render the biotechnological production economically viable again, improvements in microbial and fermentation performance are necessary. However, no comprehensive comparisons of respective species and strains used and their specific abilities exist today. Results: The genomes of a total 30 saccharolytic Clostridium strains, representative of the species Clostridium acetobutylicum, C. aurantibutyricum, C. beijerinckii, C. diolis, C. felsineum, C. pasteurianum, C. puniceum, C. roseum, C. saccharobutylicum, and C. saccharoperbutylacetonicum, have been determined; 10 of them completely, and compared to 14 published genomes of other solvent-forming clostridia. Two major groups could be differentiated and several misclassified species were detected. Conclusions: Our findings represent a comprehensive study of phylogeny and taxonomy of clostridial solvent producers that highlights differences in energy conservation mechanisms and substrate utilization between strains, and allow for the first time a direct comparison of sequentially selected industrial strains at the genetic level. Detailed data mining is now possible, supporting the identification of new engineering targets for improved solvent production. DA - 2017-03-09 DB - OpenUCT DO - 10.1186/s13068-017-0742-z DP - University of Cape Town J1 - Biotechnol Biofuels LK - https://open.uct.ac.za PB - University of Cape Town PY - 2017 T1 - Microbial solvent formation revisited by comparative genome analysis TI - Microbial solvent formation revisited by comparative genome analysis UR - http://hdl.handle.net/11427/24143 ER - en_ZA
dc.identifier.urihttp://dx.doi.org/10.1186/s13068-017-0742-z
dc.identifier.urihttp://hdl.handle.net/11427/24143
dc.identifier.vancouvercitationPoehlein A, Solano JDM, Flitsch SK, Krabben P, Winzer K, Reid SJ, et al. Microbial solvent formation revisited by comparative genome analysis. Biotechnol Biofuels. 2017; http://hdl.handle.net/11427/24143.en_ZA
dc.language.isoen
dc.publisherBioMed Central
dc.publisher.departmentDepartment of Molecular and Cell Biologyen_ZA
dc.publisher.facultyFaculty of Scienceen_ZA
dc.publisher.institutionUniversity of Cape Town
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License
dc.rights.holderThe Author(s)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceBiotechnol Biofuels
dc.source.urihttps://biotechnologyforbiofuels.biomedcentral.com/
dc.subject.otherAcetone
dc.subject.otherButanol
dc.subject.otherClostridium acetobutylicum
dc.subject.otherC. beijerinckii
dc.subject.otherC. saccharobutylicum
dc.subject.otherC. saccharoperbutylacetonicum
dc.subject.otherPhylogeny
dc.subject.otherSolvents
dc.titleMicrobial solvent formation revisited by comparative genome analysis
dc.typeJournal Article
uct.type.filetypeText
uct.type.filetypeImage
uct.type.publicationResearchen_ZA
uct.type.resourceArticleen_ZA
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